Antioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract

dc.authorid0000-0002-1487-1953en_US
dc.contributor.authorZhang, Na
dc.contributor.authorBaran, Ayşe
dc.contributor.authorValioglu, Ferzane
dc.contributor.authorTeng, Lei
dc.contributor.authorAtalar, Mehmet Nuri
dc.contributor.authorKeskin, Cumali
dc.contributor.authorWang, Xiao-Xiong
dc.contributor.authorHatipoğlu, Abdülkerim
dc.contributor.authorBaran, Mehmet Fırat
dc.contributor.authorAbdelsalam, Amine Hafis
dc.contributor.authorArslan, Şevki
dc.contributor.authorNecip, Adem
dc.date.accessioned2024-02-12T12:51:27Z
dc.date.available2024-02-12T12:51:27Z
dc.date.issued2023en_US
dc.departmentMAÜ, Fakülteler, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümüen_US
dc.description.abstractVerbascum thapsus (Mullein) is a medicinal plant used in folk medicine to treat various ailments. For this study, the biological functions of Verbascum thapsus (VT) methanol extract were determined in vitro. The plant's methanol extract was created through the maceration process. The phytochemical composition of plant extracts was investigated using liquid chromatography-electrospray ionization tandem mass spectrometry. The antioxidant capacity of the extract was determined using the 2,2-diphenyl-1-picrylhydrazil (DPPH radical) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS radical). Cell lines Caco-2 (human colorectal adenocarcinoma cells), LNCaP (Lymph Node Carcinoma of Prostate), and HEK293 (Human embryonic kidney 293 cells) were used to model colon, prostate, and non-cancerous cells. The cytotoxic activity of the plant extract on the proliferation of these cells was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay protocol. VT extract showed moderate DPPH and ABTS radical scavenging activities at 30 mg/ml concentration. With this, VT extract was determined to inhibit acetylcholinesterase (AChE) enzyme and had strong cytotoxic activity on cancerous cell lines. In addition, our findings clearly showed that the plant extract had greater cytotoxic activity on the viability of cancerous cells compared to non-cancerous (Human embryonic kidney cells; HEK293) cells. The current findings showed that V. thapsus might be a promising anti-cancer medication candidate for the treatment of human colorectal adenocarcinoma and colon cancer, as well as a good source antioxidans.en_US
dc.identifier.doi10.14715/cmb/2023.69.14.35en_US
dc.identifier.endpage216en_US
dc.identifier.issue14en_US
dc.identifier.pmid38279434en_US
dc.identifier.scopus2-s2.0-85183507957en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage211en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2023.69.14.35
dc.identifier.urihttps://hdl.handle.net/20.500.12514/5780
dc.identifier.volume69en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHatipoğlu, Abdülkerim
dc.language.isoenen_US
dc.publisherCellular and Molecular Biology Associationen_US
dc.relation.ispartofCellular and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAChE inhibitionen_US
dc.subjectAnticanceren_US
dc.subjectBioassaysen_US
dc.subjectMulleinen_US
dc.subjectPlant extracten_US
dc.titleAntioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extracten_US
dc.typeArticleen_US

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