Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley Online Library
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic
effects of extracts of C. origanifolium were investigated. In the extracts, phenolic
compound analysis by the liquid chromatography–electrospray ionization–
tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts
(HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and
human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the
GST and AChE enzyme activities of the extracts were also measured. Around 18
compounds were detected in both the methanol and ethanol extract. It was found
that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was
on methanol extract, while the ethanol extract was on Candida albicans fungus
(respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been
shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity
were found to decrease in a concentration-dependent manner.
Açıklama
Anahtar Kelimeler
Cyclotricium origanifolium
Kaynak
Chemical Biology and Drug Design
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
Sayı
Künye
Aktepe, N., Baran, A.,Atalar, M. N., Baran, F., Keskin, C., Taşkin, A.,Yavuz, Ö., Demirtaş, İ., Oğuz, E., & Jahan, I.(2022). Analysis of bioactive compounds using LC–ESI–MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium. Chemical Biology & Drug Design, 00, 1–9. https://doi.org/10.1111/cbdd.14177
