Induction of apoptosis in human hormone-refractory prostate cancer cell lines by using resveratrol in combination with AT-101

dc.authorid0000-0003-2192-9049en_US
dc.authorid0000-0003-4944-0819en_US
dc.contributor.authorAktepe, Necmettin
dc.contributor.authorYukselten, Yunus
dc.date.accessioned2021-10-21T06:19:23Z
dc.date.available2021-10-21T06:19:23Z
dc.date.issued2021en_US
dc.departmentMAÜ, Fakülteler, Sağlık Bilimleri Fakültesi, Hemşirelik Bölümüen_US
dc.description.abstractThe aim of this study was to determine the appropriate doses of AT-101 and resveratrol combination in the in vitro hormone-refractory prostate cancer (PC) cell lines, in order to evaluate the cytotoxic and genotoxic effects of this combination on the proliferation of cancer cells, namely PC-3, DU-145 and LNCAP. Cytotoxicity in PC cell lines was analysed by using the XTT Cell Proliferation Assay. DNA damage was performed with the cell death assay. Apoptotic protein levels were performed by Roche Human Apoptosis Array. IC50 values were determined by XTT analysis. The strongest combined doses (100 µM resveratrol + 5µM AT-101) were found to have the strongest synergistic apoptotic and cytotoxic effects on DU-145 cells at 72 hr. While the combined use of resveratrol and AT-101 increased the expression of markers in apoptotic cell pathways on cells, a decrease in the expression of anti-apoptotic markers was detected (p ˂ 0.05). Combined applications of these compounds showed an important synergism in the hormone-refractory PC cell lines, and it was determined that after the post-translational modification, they were significantly effective on the apoptotic pathway. These results have revealed that the combination of resveratrol and AT-101 holds great expectation as a new chemotherapeutic application in the treatment of human prostate cancer.en_US
dc.identifier.citationAktepe, N., & Yukselten, Y. (2021). Induction of apoptosis in human hormone‐refractory prostate cancer cell lines by using resveratrol in combination with AT‐101. In Andrologia. Wiley. https://doi.org/10.1111/and.14267en_US
dc.identifier.doi10.1111/and.14267en_US
dc.identifier.pmid34633104en_US
dc.identifier.scopus2-s2.0-85116724500en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1111/and.14267
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:000705332400001?AlertId=d383397b-4355-449e-9419-70f9e0e77c15&SID=F2yD84a3mpZOfw1zNMF
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85116724500&origin=resultslist&sort=plf-f&src=s&sid=490e2dd39833da2bc1ffbfd6cc1f1cd6&sot=b&sdt=b&sl=22&s=DOI%2810.1111%2fand.14267%29&relpos=0&citeCnt=0&searchTerm=
dc.identifier.urihttps://hdl.handle.net/20.500.12514/2895
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/34633104/
dc.identifier.wosWOS:000705332400001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofANDROLOGIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectapoptosis; AT-101; prostate cancer; protein array; resveratrolen_US
dc.titleInduction of apoptosis in human hormone-refractory prostate cancer cell lines by using resveratrol in combination with AT-101en_US
dc.typeArticleen_US

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