Erdem Güzel, ElifKaya Tektemur, NalanTektemur, AhmetAcay, HilalYıldırım, Ayfer2021-10-272021-10-272021Erdem Guzel, E., Kaya Tektemur, N., Tektemur, A., Acay, H., & Yildirim, A. (2021). The antioxidant and anti‐apoptotic potential of Pleurotus eryngii extract and its chitosan‐loaded nanoparticles against doxorubicin‐induced testicular toxicity in male rats. In Andrologia. Wiley. https://doi.org/10.1111/and.14225https://doi.org/10.1111/and.14225https://www.scopus.com/record/display.uri?eid=2-s2.0-85113285256&origin=SingleRecordEmailAlert&dgcid=raven_sc_affil_en_us_email&txGid=0e4006caf98943f5394e6980196ad96ahttps://hdl.handle.net/20.500.12514/2915https://www.webofscience.com/wos/woscc/full-record/WOS:000687867600001?AlertId=d383397b-4355-449e-9419-70f9e0e77c15&SID=F4vEvTomyRnE9h9jomshttps://pubmed.ncbi.nlm.nih.gov/34431122/This study was conducted to evaluate the protective role of Pleurotus eryngii extract (PE) and Pleurotus eryngii extract-loaded chitosan nanoparticles (PE-CSNP) against doxorubicin (DOX)-induced testicular toxicity in rats. Male rats were divided into six groups: control (DMSO/ethanol), PE (200 mg/kg PE), PE-CSNP (30 mg/kg PECSNP), DOX (10 mg/kg DOX, a single dose, i.p), DOX+PE (10 mg/kg DOX+200 mg/ kg PE) and DOX+PE-CSNP (10 mg/kg DOX+30 mg/kg PE-CSNP). PE and PE-CSNP were administered by oral gavage every other day for 21 days. DOX-treated rats showed histopathological impairment compared with the control group. There was an increase in the apoptotic index, caspase 3 (CASP3), BCL2-associated X apoptosis regulator (BAX), dynamin-related protein 1 (DRP1) expression and total oxidative status (TOS) in the DOX group, while mitofusin-2 (MFN2), total antioxidative status (TAS) and serum testosterone levels of the DOX group reduced when compared with the other groups. PE and PE-CSNP treatments provided significant protection against DOX-induced oxidative stress by reducing TOS levels and increasing TAS levels. CASP3, BAX, apoptotic index and DRP1-MFN2 expressions were restored by PE and PE-CSNP. However, the PE-CSNP showed higher antioxidant and anti-apoptotic efficacy compared with PE. Thus, our results provide evidence that CSNP and PE could synergistically have a potent antioxidant and anti-apoptotic therapy against DOX-induced testicular damage in male rats.eninfo:eu-repo/semantics/closedAccesschitosan nanoparticles, doxorubicin, mitochondrial dynamics, Pleurotus eryngii, testicular toxicityArticleQ3N/AWOS:0006878676000012-s2.0-8511328525634431122