Kaya Tektemur, NalanTektemur, AhmetGüzel, Elif Erdem2023-01-172023-01-172023Tektemur, N. K., TEKTEMUR, A., & Güzel, E. E. King Oyster Mushroom, Pleurotus eryngii (Agaricomycetes) Extract Can Attenuate Doxorubicin-Induced Lung Damage by Inhibiting Oxidative Stress in Rats. International Journal of Medicinal Mushrooms.https://www.scopus.com/record/display.uri?eid=2-s2.0-85143700220&origin=SingleRecordEmailAlert&dgcid=raven_sc_affil_en_us_email&txGid=23b76e23725fdfce7db306339fa9b31ehttps://hdl.handle.net/20.500.12514/3335https://pubmed.ncbi.nlm.nih.gov/36734915/Doxorubicin (DOX), a broad spectrum chemotherapeutic, has toxic effects on healthy tissues. Mitochondrial processes and oxidative stress act in the DOX-induced toxicity, therefore antioxidant therapies are widely used. The study was aimed to evaluate the therapeutic potential of Pleurotus eryngii extract (PEE), an extract of a fungus with antioxidant properties, against DOX-induced lung damage. Rats were divided into Control, DOX, DOX + PEE, and PEE groups (n = 6). DOX was administered intraperitoneally in a single dose (10 mg/kg BW) and PE (200 mg/kg BW) was administered by oral gavage every other day for 21 days. Histopathological evaluations, immunohistochemical analyses, total oxidant status (TOS)/total antioxidant status (TAS) method, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis were performed. DOX led to severe histopathological disruptions in rat lungs. Also, DOX remarkably increased the expression of dynamin 1 like (DRP1) and decreased the expression of mitofusin 1 (MFN1) and mitofusin 2 (MFN2) genes, which are related to mitochondrial dynamics. Moreover, DOX caused an increase in TOS/ TAS and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels. On the other hand, PEE treatment remarkably normalized the histopathological findings, mitochondrial dynamics-related gene expressions, markers of oxidative stress, and DNA damage. The present study signs out that PEE can ameliorate the DOX-mediated lung toxicity and the antioxidant mechanism associated with mitochondrial dynamics can have a role in this potent therapeutic effect.en10.1615/IntJMedMushrooms.2022046311info:eu-repo/semantics/closedAccessdrug toxicity, Pleurotus eryngii extract, lung, mitochondria, oxidative damage, medicinal mushroomsArticle251112N/AN/AWOS:0009056731000012-s2.0-8514370022036734915