Okumus, VeysiOzdemir, SadinKilinc, ErsinDundar, AbdurrahmanYuksel, UyanBaysal, Zubeyde14.07.20192019-07-1614.07.20192019-07-162015Okumuş, V., Özdemir, S., Kılınç, E., Dündar, A., Yüksel, U., & Baysal, Z. (2015). Preconcentration withBacillus subtilis–Immobilized Amberlite XAD-16: Determination of Cu2+and Ni2+in River, Soil, and Vegetable Samples. Bioremediation Journal, 19(1), 47–55. https://doi.org/10.1080/10889868.2014.9387241088-98681547-6529https://dx.doi.org/10.1080/10889868.2014.938724https://hdl.handle.net/20.500.12514/1379Solid-phase extraction (SPE) method was developed for the preconcentration of Cu2+ and Ni2+ before their determination by inductively coupled plasma optical emission spectrometry (ICP-OES). Bacillus subtilis-immobilized Amberlite XAD-16 was used as biosorbent. Effects of critical parameters such as pH, flow rate of samples, amount of Amberlite XAD-16 and biosorbent, sample volume, eluent type, and volume and concentration of eluent on column preconcentration of Cu2+ and Ni2+ were optimized. Applicability of the method was validated through the analysis of the certified reference tea sample (NCS ZC73014). Sensitivity of ICP-OES was improved by 36.4-fold for Cu2+ and 38.0-fold for Ni2+ by SPE-ICP-OES method. Limit of quantitation (LOQ) was found to be 0.7 and 1.1ng/ml for Cu2+ and Ni2+, respectively. Concentrations of Cu2+ and Ni2+ were determined by ICP-OES after application of developed method. Relative standard deviations (RSDs) were lower than 4.9% for Cu2+ and 7.9% for Ni2+. The Tigris River that irrigates a large agricultural part of Southeast Turkey is polluted by domestic and industrial wastes. Concentrations of Cu2+ and Ni2+ were determined in water, soil, and some edible vegetables as a biomonitor for heavy metal pollution.en10.1080/10889868.2014.938724info:eu-repo/semantics/closedAccesssensitivity improvementCu2+preconcentrationbiosorbentsolid-phase extractionNi2+Article1914755Q4Q2WOS:0003498263000052-s2.0-84924056704