Baran, AyşePolat İpek, Mehmet Fırat Baran, Ayşe Baran, Abdulkerim Hatipoğlu, Cumali Keskin, Mahmut Yildiztekin, Selçuk Küçükaydin, Hatice Becerekli, Kadri Kurt, Aziz Eftekhari, Irada Huseynova, Rovshan Khalilov, William C. Cho2023-08-022023-08-022023İpek, P., Baran, M. F., Baran, A., Hatipoğlu, A., Keskin, C., Yildiztekin, M., ... & Cho, W. C. (2023). Green synthesis and evaluation of antipathogenic, antioxidant, and anticholinesterase activities of gold nanoparticles (Au NPs) from Allium cepa L. peel aqueous extract. Biomass Conversion and Biorefinery, 1-10.https://doi.org/10.1007/s13399-023-04362-yhttps://www.scopus.com/record/display.uri?eid=2-s2.0-85160849619&origin=SingleRecordEmailAlert&dgcid=raven_sc_affil_en_us_email&txGid=b84993f3b60bff5315682c0588c8228dhttps://hdl.handle.net/20.500.12514/3574Gold nanoparticles (Au NPs) have potentially therapeutic properties as they are synthesized via biomolecules as reducing and stabilizing agent(s). The aim of this study is to develop an easy and eco-friendly method for the synthesis of Au NPs using extracts from the Allium cepa (AC) red peel (skin) extract and investigate the antibacterial and antioxidant activity and also inhibitory efects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. UV–Vis peak at~564 nm confrmed the Au NPs absorbance. TEM images revealed the formation of Au NPs with mostly spherical shapes and sizes between 6.08 and 54.20 nm. FTIR analysis confrmed the important biological compounds responsible for the reduction of gold. The strong absorption property of Au NPs was studied by EDX. The produced Au NPs demonstrated signifcant antibacterial and antifungal activity against the bacterial and fungal strains tested, as well as efcient inhibitory activity against both AChE and BChE enzymes. The highest antimicrobial activities were found against Staphylococcus aureus (0.06° mg/ ml) and Candida albicans (0.06° mg/ml). The antioxidant test fndings revealed that AC-Au NPs had lesser activity when compared to normal antioxidants. The Au NPs showed excellent inhibitory efcacy against AChE and BChE. The proposed green technique could encourage the innocuous generation of Au NPs, implying therapeutic possibilities.en10.1007/s13399-023-04362-yinfo:eu-repo/semantics/closedAccessAllium cepa · Antioxidant · Anticholinesterase activity · Gold nanoparticles · Plant-based nanoparticlesArticleN/AN/AWOS:0010003230000012-s2.0-85160849619