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dc.contributor.authorOzdemir, Sadin
dc.contributor.authorOduncu, M. Kadir
dc.contributor.authorKilinc, Ersin
dc.contributor.authorSoylak, Mustafa
dc.identifier.citationOzdemir, S., Oduncu, M. K., Kilinc, E., & Soylak, M. (2017). Resistance, bioaccumulation and solid phase extraction of uranium (VI) by Bacillus vallismortis and its UV–vis spectrophotometric determination. Journal of Environmental Radioactivity, 171, 217–225.
dc.descriptionWOS: 000399510500023en_US
dc.descriptionPubMed ID: 28285087en_US
dc.description.abstractBioaccumulation, resistance and preconcentration of uranium(VI) by thermotolerant Bacillus vallismortis were investigated in details. The minimum inhibition concentration of (MIC) value of U(VI) was found as 85 mg/L and 15 mg/L in liquid and solid medium, respectively. Furthermore, the effect of various U(VI) concentrations on the growth of bacteria and bioaccumulation on B. vallismortis was examined in the liquid culture media. The growth was not significantly affected in the presence of 1.0, 2.5 and 5.0 mg/L U(VI) up to 72 h. The highest bioaccumulation value at 1 mg/L U(VI) concentration was detected at the 72nd hour (10 mg/g metal/dry bacteria), while the maximum bioaccumulation value at 5 mg/L U(VI) concentration was determined at the 48th hour (50 mg metal/dry bacteria). In addition to these, various concentration of U(VI) on alpha-amylase production was studied. The alpha-amylase activities at 0, 1, 2.5 and 5 mg/L U(VI) were found as 3313.2, 3845.2, 3687.1 and 3060.8 U/mg, respectively at 48th. Besides, uranium (VI) ions were preconcentrated with immobilized B. vallismortis onto multiwalled carbon nanotube (MWCNT) and were determined by UV-vis spectrophotometry. The surface macro structure and functionalities of B. vallismortis immobilized onto multiwalled carbon nanotube with and without U(VI) were examined by FT-IR and SEM. The optimum pH and flow rate for the biosorption of U(VI) were 4.0-5.0 and 1.0 mL/min, respectively. The quantitative elution occurred with 5.0 mL of 1 mol/L HCI. The loading capacity of immobilized B. vallismortis was determined as 23.6 mg/g. The certified reference sample was employed for the validation of developed solid phase extraction method. The new validated method was applied to the determination of U(VI) in water samples from Van Lake-Turkey. (C) 2017 Elsevier Ltd. All rights reserved.en_US
dc.publisherELSEVIER SCI LTDen_US
dc.subjectBacillus vallismortisen_US
dc.titleResistance, bioaccumulation and solid phase extraction of uranium (VI) by Bacillus vallismortis and its UV-vis spectrophotometric determinationen_US
dc.departmentMAÜ, Meslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.authoridSoylak, Mustafa -- 0000-0002-1017-0244; Kilinc, Ersin -- 0000-0001-5223-9919en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US

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