Yazar "Soylak, Mustafa" seçeneğine göre listele

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    Boletus edulis loaded with gamma-Fe2O3 nanoparticles as a magnetic sorbent for preconcentration of Co(II) and Sn(II) prior to their determination by ICP-OES
    (SPRINGER WIEN, 2018) Özdemir, Sadin; Yalçın, M. Serkan; Kılınç, Ersin; Soylak, Mustafa
    The authors show that the fungus Boletus edulis loaded with gamma-Fe2O3 nanoparticles is a viable sorbent for magnetic solid phase extraction of trace levels of Co(II) and Sn(II). The surface structure of immobilized magnetized B. edulis was characterized by FT-IR, SEM and EDX. Experimental parameters were optimized. Following elution with 1 M HCl, the ions were quantified by ICP-OES. The limits of detection are 21 pg.mL(-1) for Co(II), and 19 pg.mL(-1) for Sn(II). The preconcentration factors are 100 for both ions. The sorption capacities of the sorbent are 35.8 mg.g(-1) for Co(II) and 29.6 mg.g(-1) for Sn(II). The method was applied to the analysis of certificated reference materials and gave >= 95% recoveries with low RSDs. It was also successfully applied to the quantification of Co(II) and Sn(II) in spiked environmental and food samples.
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    Comparative solid phase extraction study on the U(VI) preconcentration by using immobilized thermotolerant Bacillus vallismortis and Bacillus mojavensis
    (SPRINGER, 2018) Ozdemir, Sadin; Oduncu, M. Kadir; Kilinc, Ersin; Soylak, Mustafa
    Bacillus vallismortis and Bacillus mojavensis were loaded onto Amberlite XAD-4 resin and used for solid phase extraction (SPE) of uranium(VI). A quick and simple UV-Vis spectrophotometric method was used to determine U(VI) ion. The best experimental conditions were determined as being a pH of 5.0; a sample flow rate of 2.0 mL min(-1); 200.0 mg of biosorbent; 800 mg of Amberlite XAD-4, and 5.0 mL of 1 mol L-1 HCl as desorption solution for both immobilized bacteria. The preconcentration factors were achieved as 80 for both solid phase extractor. The developed methods were validated by applying to reference water and tea samples.
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    Development of Armillae mellea immobilized nanodiamond for the preconcentrations of Cr(III), Hg(II) and Zn(II)
    (Analytical Biochemistry, 2021) Kılınç, Ersin; Ozdemir, Sadin; Şen, Fatih; Soylak, Mustafa
    In this study, we present an environmental friend and easy procedure for simultaneous preconcentration of Cr(III), Hg(II) and Zn(II) by solid-phase extraction before their determination by inductively coupled plasma optical emission spectrometry. Armillae mellea immobilized nanodiamond was used as sorbent. During the study, critical parameters influencing the extraction performance were investigated in detail. The best parameters were found as pH 5.0, 2.0 mL min−1 of flow rate, 200 mg of Armillae mellea, 300 mL of sample volume. LOD values were found as 0.025, 0.13 and 0.038 ng mL−1, respectively for Cr(III), Hg(II) and Zn(II). By applying the developed procedure, sensitivities of ICP-OES were improved for 60 fold for Cr(III), Hg(II) and Zn(II). Their concentrations in different food samples were measured after microwave digestion and solid-phase extraction.
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    Magnetic solid phase extractions of Co(II) and Hg(II) by using magnetized C-micaceus from water and food samples
    (ELSEVIER SCI LTD, 2019) Ozdemir, Sadin; Mohamedsaid, Siham Abdullah; Kilinc, Ersin; Soylak, Mustafa
    A new bio-MSPE sorbent based on the use of C. micaceus and gamma-Fe2O3 magnetic nanoparticle was prepared for the preconcentrations of Co(II) and Hg(II). Critical parameters including pH, flow rate, quantity of C. micaceus, quantity of gamma-Fe2O3 magnetic nanoparticle, eluent (type, concentration and volume), sample volume, and foreign ions were examined. Surface structure and variations after interaction with Co(II) and Hg(II) of bio-MSPE sorbent were investigated by FT-IR, SEM, and EDX. The impact of bio-MSPE column reusage was also tested. The biosorption capacities were determined as 24.7 mg g(-1) and 26.2 mg g(-1), respectively for Co(II) and Hg(II). Certified reference materials were utilized to find out the accuracy of the prepared bio-MSPE method. This novel bio-MSPE method was accomplished by being applied to real food and water samples. In particular, it will be possible to make use of C. micaceus as new alternatives, in environmental biotechnology applications.
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    Resistance, bioaccumulation and solid phase extraction of uranium (VI) by Bacillus vallismortis and its UV-vis spectrophotometric determination
    (ELSEVIER SCI LTD, 2017) Ozdemir, Sadin; Oduncu, M. Kadir; Kilinc, Ersin; Soylak, Mustafa
    Bioaccumulation, resistance and preconcentration of uranium(VI) by thermotolerant Bacillus vallismortis were investigated in details. The minimum inhibition concentration of (MIC) value of U(VI) was found as 85 mg/L and 15 mg/L in liquid and solid medium, respectively. Furthermore, the effect of various U(VI) concentrations on the growth of bacteria and bioaccumulation on B. vallismortis was examined in the liquid culture media. The growth was not significantly affected in the presence of 1.0, 2.5 and 5.0 mg/L U(VI) up to 72 h. The highest bioaccumulation value at 1 mg/L U(VI) concentration was detected at the 72nd hour (10 mg/g metal/dry bacteria), while the maximum bioaccumulation value at 5 mg/L U(VI) concentration was determined at the 48th hour (50 mg metal/dry bacteria). In addition to these, various concentration of U(VI) on alpha-amylase production was studied. The alpha-amylase activities at 0, 1, 2.5 and 5 mg/L U(VI) were found as 3313.2, 3845.2, 3687.1 and 3060.8 U/mg, respectively at 48th. Besides, uranium (VI) ions were preconcentrated with immobilized B. vallismortis onto multiwalled carbon nanotube (MWCNT) and were determined by UV-vis spectrophotometry. The surface macro structure and functionalities of B. vallismortis immobilized onto multiwalled carbon nanotube with and without U(VI) were examined by FT-IR and SEM. The optimum pH and flow rate for the biosorption of U(VI) were 4.0-5.0 and 1.0 mL/min, respectively. The quantitative elution occurred with 5.0 mL of 1 mol/L HCI. The loading capacity of immobilized B. vallismortis was determined as 23.6 mg/g. The certified reference sample was employed for the validation of developed solid phase extraction method. The new validated method was applied to the determination of U(VI) in water samples from Van Lake-Turkey. (C) 2017 Elsevier Ltd. All rights reserved.
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    Simultaneous preconcentrations of Co2+, Cr6+, Hg2+ and Pb2+ ions by Bacillus altitudinis immobilized nanodiamond prior to their determinations in food samples by ICP-OES
    (ELSEVIER SCI LTD, 2017) Ozdemir, Sadin; Kilinc, Ersin; Celik, Kadir Serdar; Okumus, Veysi; Soylak, Mustafa; ;
    A novel solid phase extraction method was developed for simultaneous preconcentration-separation of Co2+, Cr6+, Hg2+ and Pb2+ ions prior to their determinations in food samples by ICP-OES. Thermophilic Bacillus altitudinis immobilized nanodiamond was used as a new biosorbent. SEM and FT-IR analysis were studied to characterize the biosorbent. The optimum pH values of quantitative biosorption for Co2+, Cr6+, Hg2+ and Pb2+ were found to be 5.0, 6.0, 6.0 and 6.0, respectively. A flow rate of 3.0 mL min(-1) was selected as optimum for all metal ions. 5 mL of 1 mol/L HCl was used as eluent. Preconcentration factor was achieved as 80. LODs were calculated as 0.071, 0.023, 0.016 and 0.034 ng mL(-1), respectively for Hg2+, Co2+, Cr6+ and Pb2+. The biosorption capacities were calculated for Co2+, Cr6+, Hg2+ and Pb2+ as 26.4, 30.4, 19.5, and 35.2 mg/g, respectively. The developed method was successfully applied to food samples to determine analyte concentrations. (C) 2016 Published by Elsevier Ltd.
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    Tolerance and bioaccumulation of U(VI) by Bacillus mojavensis and its solid phase preconcentration by Bacillus mojavensis immobilized multiwalled carbon nanotube
    (ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2017) Ozdemir, Sadin; Oduncu, M. Kadir; Kilinc, Ersin; Soylak, Mustafa
    In this study, uranium(VI) tolerance and bioaccumulation were investigated by using thermo-tolerant Bacillus mojavensis. The level of U(VI) was measured by UV-VIS spectrophotometry. The minimum inhibition concentration (MIC) value of U(VI) was experimented. Bacterial growth was not affected in the presence of 1.0 and 2.5 mg/L U(VI) at 36 h and the growth was partially affected in the presence of 5 mg/L U(VI) at 24 h. What was obtained from this study is that there was diversity in the various periods of the growth phases of metal bioaccumulation capacity, which was shown by B. mojavensis. The maximum bioaccumulation capacities were found to be 12.8, 22.7, and 48.2 mg/g dried bacteria, at 24th hours at concentration of 1.0, 2.5 and 5 mg/L U(VI), respectively. In addition to these, U(VI) has been pre-concentrated on B. mojavensis immobilized MWCNT. Several factors such as pH, flow rate of solution, amount of biosorbent and support materials, eluent type, concentration and volume, the matrix interference effect on retention have been studied, and extraction conditions were optimized. Preconcentration factor was achieved as 60. Under the optimized conditions, the limit of detection (LOD) and quantification (LOQ) were calculated as 0.74 and 2.47 mu g/L. The biosorption capacity of immobilized B. mojavensis was calculated for U(VI) as 25.8 mg/g. The results demonstrated that the immobilized biosorbent column could be reused at least 30 cycles of biosorption and desorption with the higher than 95% recovery. FT-IR and SEM analysis were performed to understand the surface properties of B. mojavensis. (C) 2016 Elsevier Ltd. All rights reserved.