Fungi isolated from cankered tissues of declining apricot trees in Malatya and Elazığ provinces of Turkey
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Dosyalar
Tarih
Kasım, 2017
Dergi Başlığı
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Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Surveys were carried out in apricot (Prunus armeniaca L.) production areas of Malatya and
Elazığ provinces from April to November in 2015 and 2016. Fungal and oomycetous diseases
causing dieback and decline symptoms were investigated and locations where the diseases
were prevalent were determined according to the districts in these provinces. Nine and 40
orchards were visited in Elazığ and Malatya during the course of the surveys. A total of 665
out of 5750 apricot trees were checked and the disease incidence was found to be 44% in
the surveyed orchards. Out of isolates obtained from root and crown tissues of symptomatic
trees, isolates obtained from cankered tissues were characterized according to their
morphological characteristics. Genomic DNA was extracted from representative isolates. The
internal transcribed spacer (ITS) region of rDNA was amplified using the ITS6/ITS4 primer
pair and sequenced and submitted to GenBank. NCBI BLAST results showed 98 to 100%
similarity with the ITS sequences of many Clonostachys rosea f. rosea (Link : Fr.) Schroers et.
al. 1999 (Ascomycetes, Hypocreales), Sarocladium kiliense (Grütz) Summerb. 2011
(Ascomycetes, Incertae sedis) (Syn: Acremonium kiliense), Phoma sp. (Ascomycetes,
Pleosporales), Entoleuca spp. (Ascomycetes, Xylariales) strains deposited in NCBI GenBank.
The sequences were submitted to GenBank and given accession numbers were MF536537
and MF536538 for C. rosea, MF536539 for S. kiliense, MF536540 and MF536541 for Phoma
spp., and MF536542, MF536543, MF536544 and MF536545 for Entoleuca spp. isolates.
Moreover, Verticillium dahliae and Macrophomina phaseolina were also isolated from inner
tissues of necrotic branches and morphologically identified. However, pathogenicity of these
isolates needs further investigations. If some isolates were not pathogenic, their endophytic
or hperparasitic characteristics against pathogenic ones should be tested in order to fully
exploit their potential for use as biological control agents.
Açıklama
Anahtar Kelimeler
Prunus armeniaca L., Canker, Fungi.
Kaynak
II. International Iğdır Symposium
WoS Q Değeri
Scopus Q Değeri
Cilt
1
