Effects of Concomitant Use of N-acetylcysteine and Cyclosporine A on Acetaminophen-induced Acute Kidney Injury in Mice

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dc.contributor.authorOzan Kocamüftüoğlu, Gonca
dc.contributor.authorKaya Tektemür, Nalan
dc.contributor.authorErdem Güzel, Elif
dc.contributor.authorTektemur, Ahmet
dc.contributor.authorOzan, İbrahim Enver
dc.date.accessioned2024-04-05T08:32:52Z
dc.date.available2024-04-05T08:32:52Z
dc.date.issued2024
dc.departmentMAÜ, Fakülteler, Sağlık Bilimleri Fakültesi, Ebelik Bölümü
dc.description.abstractBackground: Acetaminophen (APAP), a commonly used analgesic, causes acute kidney injury (AKI) in overdose although it is rare. Mitochondrial dysfunction plays a major role in the pathophysiology of renal damage, although the exact molecular mechanism is unknown. This study aimed to evaluate the potential therapeutic effect of cyclosporin A (CsA), a mitochondrial membrane permeability transition pore (MPTP) inhibitor, with N-acetylcysteine (NAC) in APAP-induced AKI. Methods: Male BALB/c mice were divided into Control, APAP, APAP+NAC, APAP+CsA and APAP+NAC+CsA groups (n=6). A single dose of APAP (400 mg/kg) was administered intraperitoneally. All other treatments (1200 mg/kg NAC, 50 mg/kg CsA) were performed intraperitoneally 3 h after APAP administration. All animals were decapitated and blood samples and kidney tissue samples were collected for evaluation. Serum creatinine (Cr) and blood urea nitrogen (BUN) levels were measured. The kidney tissue 8-hydroxydeoxyguanosine (8-OHdG), cytochrome c (Cytc) and 3-nitrotyrosine (3-NT) levels and cytochrome c (Cytc) expressions were determined. Result: Increased Cr and BUN levels, histopathological examinations and expressions of 8-OHdG, 3-NT and Cytc were detected in the APAP group. Combined NAC+CsA treatment sufficiently reversed oxidative stress, serum Cr and BUN levels and histopathological alterations induced by APAP. Moreover, cytc levels and renal tubular injury were remarkably reduced by combined drug treatment compared to the APAP+NAC group. These data suggest that the therapeutic effect of combined NAC+CsA treatment in mice with APAP-induced nephrotoxicity can be related to the combination of the antioxidant effect of NAC and the mitochondrial MPTP inhibitor effect of CsA.
dc.identifier.citationKocamüftüoğlu, G. O., Tektemur, N. K., Güzel, E. E., Tektemur, A., & Ozan, İ. E. (2024). Effects of concomitant use of n-acetylcysteine and cyclosporine a on acetaminophen-induced acute kidney injury in mice. Indian Journal of Animal Research, (Of). https://doi.org/10.18805/ijar.bf-1736
dc.identifier.doi10.18805/IJAR.BF-1736
dc.identifier.endpage469
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85188805210
dc.identifier.scopusqualityQ3
dc.identifier.startpage459
dc.identifier.urihttps://doi.org/10.18805/ijar.bf-1736
dc.identifier.urihttps://hdl.handle.net/20.500.12514/5856
dc.identifier.volume58
dc.identifier.wosqualityN/A
dc.institutionauthorErdem Güzel, Elif
dc.institutionauthorid0000-0002-2097-7818
dc.language.isoen
dc.publisherAgricultural Research Communication Centre
dc.relation.ispartofIndian Journal of Animal Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject3-nitrotyrosine
dc.subject8-Hydroxy-deoxyguanosine
dc.subjectCombined drug treatment
dc.subjectCytochrome c
dc.subjectMitochondrial dysfunction
dc.subjectNephrotoxicity
dc.titleEffects of Concomitant Use of N-acetylcysteine and Cyclosporine A on Acetaminophen-induced Acute Kidney Injury in Mice
dc.typeArticle

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