Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital

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dc.authoridorcid.org/ 0000-0002-6365-0196en_US
dc.contributor.authorDemir Cemil
dc.contributor.authorAslantas, Ozkan
dc.contributor.authorDuran, Nızamı
dc.contributor.authorOcak, S
dc.contributor.authorÖzer, Burcın
dc.date.accessioned2020-01-07T20:15:44Z
dc.date.available2020-01-07T20:15:44Z
dc.date.issuedNisan 2011en_US
dc.departmentMAÜ, Meslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.description.abstractAim: The aim of this study was to investigate the presence of genes encoding staphylococcal enterotoxins (SEs), exfoliative toxins (ETAs, ETBs), and toxic shock syndrome toxin-1 (TSST-1) by polymerase chain reaction (PCR) in Staphylococcus aureus strains isolated from various clinical samples from the Mustafa Kemal University Hospital. In addition, PCR-based restriction fragment length polymorphism (RFLP) analysis of the coa gene was employed to genotype the isolates. Materials and methods: A total of 120 S. aureus strains isolated from various clinical samples (blood, wounds, urine, conjuctival swabs, and tracheal aspirate) over a 1 year period, 2007-2008, were used in this study. Results: Almost 65.8% of the isolates possessed at least one toxin gene. The genes most frequently found were seg-sei (40.8%), followed by sea (30%) and eta (19.2%). Overall, 35 toxin genotypes were observed, among which the genotypes seg-sei, sea-seg-sei, and sea-see predominated at the rate of 8.3%, 5.8%, and 5%, respectively. Four coagulase genotype patterns were observed, with molecular sizes ranging from 570 to 970 bp. Coo-based RFLP analysis revealed 7 different patterns using Conclusion: Our results have revealed that toxin genes were very prevalent among S. aureus isolates, and the toxigenic isolates were independent of the genotypes obtained by PCR-RFLP of the coa gene (P > 0.05).en_US
dc.identifier.doi10.3906/sag-1003-657en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12514/2106
dc.language.isoenen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Başka Kurum Yazarıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStaphylococcus aureus; toxin genes; PCR-RFLP; coagulaseen_US
dc.titleInvestigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospitalen_US
dc.typeArticleen_US

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