An enzyme-linked immunosorbent assay for brucella specific antibody and real-time PCR for detecting Brucella spp. in milk and cheese in Sanliurfa, Turkey

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dc.contributor.authorAltun S.K.
dc.contributor.authorYigin A.
dc.contributor.authorGürbilek S.E.
dc.contributor.authorGürbüz S.
dc.contributor.authorDemirci M.
dc.contributor.authorKeskin O.
dc.contributor.authorTel O.Y.
dc.date.accessioned14.07.201910:50:10
dc.date.accessioned2019-07-16T20:42:34Z
dc.date.available14.07.201910:50:10
dc.date.available2019-07-16T20:42:34Z
dc.date.issued2017
dc.department[Belirlenecek]en_US
dc.description.abstractThe objective of this study was to investigate the presence of anti-Brucella antibody and Brucella spp. DNA in cow, sheep and goat milk and in Urfa cheese collected from markets and bazaars in Sanliurfa, located in southeast of Turkey. A total of 258 samples consisting of 178 raw milk (48 cow milk, 65 sheep milk and 65 goat milk) samples and 80 Urfa cheese samples were investigated. Anti-Brucella antibody was detected by indirect ELISA (i-ELISA), and the presence of Brucella spp. DNA was screened by real time Polymerase Chain Reaction (RT-PCR). 16.6% of the cow, 6.1% of the goat and 6.1% of the sheep milk and 16.25% of the cheese samples were found as positive for brucella antibodies by i-ELISA. The RT-PCR assay amplified Brucella DNA from 18.75, 7.6 and 6.1% cow, goat and sheep milk samples respectively. Brucella DNA was amplified from 22.5% cheese samples. The 11.2% and 13.9% of the samples were found as positive by i-ELISA and RT-PCR respectively. This study indicates that milk and milk products consumed in Sanliurfa poses a risk to public health in terms of brucellosis. The combining usage of both i-ELISA and RT-PCR methods could lead to more reliable results to detect anti-Brucella antibody and Brucella spp. DNA from milk and cheese samples. © 2016 PVJ.en_US
dc.identifier.endpage42en_US
dc.identifier.issn0253-8318
dc.identifier.issue1en_US
dc.identifier.startpage39en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12514/1156
dc.identifier.volume37en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniversity of Agricultureen_US
dc.relation.ispartofPakistan Veterinary Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBrucellaen_US
dc.subjectCheeseen_US
dc.subjectI-ELISAen_US
dc.subjectMilken_US
dc.subjectPCRen_US
dc.titleAn enzyme-linked immunosorbent assay for brucella specific antibody and real-time PCR for detecting Brucella spp. in milk and cheese in Sanliurfa, Turkeyen_US
dc.typeArticleen_US

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