An enzyme-linked immunosorbent assay for brucella specific antibody and real-time PCR for detecting Brucella spp. in milk and cheese in Sanliurfa, Turkey
dc.contributor.author | Altun S.K. | |
dc.contributor.author | Yigin A. | |
dc.contributor.author | Gürbilek S.E. | |
dc.contributor.author | Gürbüz S. | |
dc.contributor.author | Demirci M. | |
dc.contributor.author | Keskin O. | |
dc.contributor.author | Tel O.Y. | |
dc.date.accessioned | 14.07.201910:50:10 | |
dc.date.accessioned | 2019-07-16T20:42:34Z | |
dc.date.available | 14.07.201910:50:10 | |
dc.date.available | 2019-07-16T20:42:34Z | |
dc.date.issued | 2017 | |
dc.department | [Belirlenecek] | en_US |
dc.description.abstract | The objective of this study was to investigate the presence of anti-Brucella antibody and Brucella spp. DNA in cow, sheep and goat milk and in Urfa cheese collected from markets and bazaars in Sanliurfa, located in southeast of Turkey. A total of 258 samples consisting of 178 raw milk (48 cow milk, 65 sheep milk and 65 goat milk) samples and 80 Urfa cheese samples were investigated. Anti-Brucella antibody was detected by indirect ELISA (i-ELISA), and the presence of Brucella spp. DNA was screened by real time Polymerase Chain Reaction (RT-PCR). 16.6% of the cow, 6.1% of the goat and 6.1% of the sheep milk and 16.25% of the cheese samples were found as positive for brucella antibodies by i-ELISA. The RT-PCR assay amplified Brucella DNA from 18.75, 7.6 and 6.1% cow, goat and sheep milk samples respectively. Brucella DNA was amplified from 22.5% cheese samples. The 11.2% and 13.9% of the samples were found as positive by i-ELISA and RT-PCR respectively. This study indicates that milk and milk products consumed in Sanliurfa poses a risk to public health in terms of brucellosis. The combining usage of both i-ELISA and RT-PCR methods could lead to more reliable results to detect anti-Brucella antibody and Brucella spp. DNA from milk and cheese samples. © 2016 PVJ. | en_US |
dc.identifier.endpage | 42 | en_US |
dc.identifier.issn | 0253-8318 | |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 39 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12514/1156 | |
dc.identifier.volume | 37 | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Agriculture | en_US |
dc.relation.ispartof | Pakistan Veterinary Journal | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Brucella | en_US |
dc.subject | Cheese | en_US |
dc.subject | I-ELISA | en_US |
dc.subject | Milk | en_US |
dc.subject | PCR | en_US |
dc.title | An enzyme-linked immunosorbent assay for brucella specific antibody and real-time PCR for detecting Brucella spp. in milk and cheese in Sanliurfa, Turkey | en_US |
dc.type | Article | en_US |