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Öğe Can Serum Biomarker Values from Second-Trimester Aneuploidy Screening Predict the development of Retinopathy of Prematurity in Premature Infants?(Kare Publishing, 2024) Sabancı, Şenol; Küçük, Mehmet Fatih; Savaş, Hasan Basri; Süren, Elçin; Erol, Muhammet Kazım; Yavuz, And; Sipahioğlu, HaydarObjectives: This study aimed to evaluate serum biomarker values measured during second-trimester aneuploidy screening in terms of their predictive ability for the development of retinopathy of prematurity (ROP) in premature infants. Methods: This retrospective cohort study evaluated the data of 1985 idiopathic premature infants who underwent ROP screening from 2016 to 2022. The infants were divided into two groups according to the presence of ROP, and those with ROP were further evaluated in two subgroups based on the presence of proliferation. Comparisons were made concerning the serum multiple of the median values of unconjugated estriol (uE3), human chorionic gonadotropin (hCG), and alpha-fetoprotein (AFP) among aneuploidy screening biomarkers. Results: While 1628 premature infants were in the non-ROP group, 357 were in the ROP group. Of the infants with ROP, 72 were in the proliferative ROP group and 285 in the non-proliferative ROP group. There was no significant difference in the multiple of the median values of the evaluated serum biomarkers (uE3, hCG, and AFP) between the ROP and non-ROP groups or between the proliferative ROP, non-proliferative ROP, and non-ROP groups. Conclusion: The multiple of the median values of second-trimester aneuploidy screening serum biomarkers were not able to predict the development of ROP in premature infants. This result may have been caused by the fact that the blood tests were taken only once and in the same weeks.Öğe Comparison of growth factor levels in injectable platelet-rich fibrin obtained from healthy individuals and patients with chronic periodontitis: a pilot study(Springer, 2024) Karcı, Bilge; Savaş, Hasan BasriBackground: This study aimed to assess and compare the concentrations of growth factors, white blood cells (WBCs), and platelets in injectable platelet-rich fibrin (i-PRF) derived from people with healthy periodontal conditions and those with chronic periodontitis. Methods: Venous blood samples were obtained from 30 patients diagnosed with chronic periodontitis (test group) and 30 participants with healthy periodontal conditions (control group). The i-PRF was then acquired from centrifuged blood. The growth factors (VEGF, IGF-1, TGF-β1, PDGF-BB and EGF) released from the i-PRF samples were compared between groups with ELISA testing. The amounts of WBCs and platelets were also compared. Results: No significant differences in the concentrations of growth factors were found between the groups (the mean values for the control and test groups were, respectively: IGF: 38.82, 42.46; PDGF: 414.25, 466.28; VEGF: 375.69, 412.18; TGF-β1: 21.50, 26.21; EGF: 138.62, 154.82). The test group exhibited a significantly higher WBC count than the control group (8.80 vs. 6.60, respectively). However, the platelet count did not show a statistically significant difference between the groups (control group 242.0 vs. test group 262.50). No significant correlation was observed between WBC count and growth factor level in either group. Conclusions: The growth factor levels in i-PRFs did not exhibit significant difference between the two groups. This suggests that the levels of these growth factors may be unaffected by the periodontal disease.Öğe The protective role of selenium in an experimental high fructose corn syrup exposure.(Modestum, 2023)Objective: Nowadays, fructose is recognized as a significant health threat. Prepared foods containing fructose are consumed more because they do not create a feeling of satiety. Selenium is an essential trace element with antioxidant and cell protective properties. In this study, the effect of high fructose corn syrup, which is used as a sweetener in many foods and beverages and consumed during pregnancy, and the possible protective role of selenium in this effect were investigated and examined. Methods: Fertilized specific pathogen-free eggs were used in our study. These eggs were divided equally into four groups. Each group was allocated 10 eggs containing viable embryos. These groups are pre-process control, post-process control, high fructose corn syrup (HFCS55), and high fructose corn syrup and selenium (HFCS-55+Se 10-6 ) groups. Pellets containing and containing no active substance (HFCS-55, HFCS-55+Se 10-6 ) were carefully placed on the chorioallantoic membrane (CAM) of each egg in these groups. Oxidative stress status in all groups was determined by total oxidative stress (TOS) and total antioxidant capacity (TAC) methods. Results: In our study, a significant increase in TOS levels and a significant decrease in TAC levels were observed in egg groups given HFCS compared to other groups (p<0.05). The OSI value was shown to be lower in the group given HFCS+Se. Conclusion: As a result, HFCS was shown to increase oxidative stress. In line with our data, it has been shown that Se, plays a protective role against oxidative stress.Öğe Anti-angiogenic and oxidative effects of sodium benzoate at different concentrations in chorioallantoic membrane model.(Modestum, 2023)Aim: Due to the increased consumption of packaged foods, exposure to food additives is also increasing. Sodium benzoate (SB), a frequently used food additive, is generally used in alcoholic beverages, fruit, and vegetable juices, carbonated soft drinks, canned food, and various sauces. It is used to inhibit the formation of mold, yeast, and bacteria. This study was carried out to investigate the effects of SB exposure on angiogenesis and oxidant-antioxidant balance. Materials & methods: Three different concentrations of SB, bevacizumab, and empty pellets were prepared, placed on chorioallantoic membrane (CAM), and examined for antiangiogenesis. Total antioxidant capacity (TAC) and total oxidant capacity (TOC) measurements were made in the albumen samples, and oxidative stress index (OSI) value was calculated. Results: The control group had no anti-angiogenic effect, but the bevacizumab group had a strong anti-angiogenic effect.10-3 M SB had a weak anti-angiogenic effect, but 10-4 M SB and 10-5 M SB showed no anti-angiogenic effect. TOC levels increased with SB in a dose-dependent manner. TAC levels decreased depending on the dose in the experimental groups with SB application. OSI levels increased depending on the dose increase in SB. Conclusions: SB exposure caused a dose-dependent increase in oxidative stress and antiangiogenic effect in CAM model.Öğe Anti-angiogenic and oxidative effects of brilliant blue at different concentrations in chorioallantoic membrane model(Modestum, 2023)Introduction: Artificial food colorings are increasingly used to make the color and appearance of foods more attractive. One commonly used food dye is E133, international-coded brilliant blue (BB). According to the European Food Safety Authority panel results in 2010, it was determined that BB daily remained below the acceptable daily intake (ADI) value for adults and exceeded ADI value for children. The purpose of this study was to investigate the effects of BB on angiogenesis and oxidative stress in the chorioallantoic membrane model. Materials & methods: In this investigation, fertilized chick eggs free of specific pathogens were used. The eggs that were not fully grown or fertilized were excluded. 50 embryos were distributed into five groups of 10 each. The negative control group was the control group, the positive control group was the bevacizumab group, and three different BB dosages (10-4 M, 10-5 M, and 10-6 M) were identified. At the end of the experiment, anti-angiogenesis scoring, and total antioxidant-oxidant capacity were evaluated. Results: According to the average score values, the control group had no anti-angiogenic impact, but the bevacizumab group had a strong anti-angiogenic effect (average score 1.1). Furthermore, the 10-4 M BB group had a weak anti-angiogenic impact (average score of 0.7), while the 10-5 M and 10-6 M BB groups had no anti-angiogenic effect (average score of 0.4 and 0.2, respectively). As a result of one-way analysis of variance test, it was seen that BB significantly increased total oxidant capacity and oxidative stress index values in proportion to the increase in dose (p<0.05). Conclusions: BB’s oxidant and anti-angiogenic effects indicate that high doses of processed foods containing artificial food dyes carry a risk for viable growth. Since there are not enough studies in the literature showing the oxidant or antiangiogenic effects of BB in chorioallantoic membrane model, the original data we presented in this study are pioneering.Öğe Öğe Öğe Anti-Angiogenic and Oxidant Effects of Monosodium Glutamate at Different Concentrations in Chorioallantoic Membrane Model(Muğla Sıtkı Koçman Üniversitesi, 21.03.2023)Monosodium glutamate (MSG) is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG (10-4 M, 10-5 M, and 10-6 M), control, and the bevacizumab (10-6 M) were prepared and placed on the chorioallantoic membrane (CAM) of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group (average score: 0.3). Anti-angiogenic effects were moderate in the 10-5 M MSG group (average score: 0.5) and in the 10-4 M MSG group (average score: 0.7), and strong in the bevacizumab group (average score: 1.1). According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.Öğe Is There a Relationship Between First-Trimester Aneuploidy Screening Serum Biomarker Values and Nuchal Translucency Measurements and the Development of Retinopathy of Prematurity (ROP) in Premature Infants?(Nature, 10/13/2023)Abstract Purpose The purpose of this study is the evaluation of serum biomarker and nuchal translucency (NT) values measured during first-trimester aneuploidy screening in terms of the development of retinopathy of prematurity (ROP) in premature infants and investigation of whether the development of ROP is associated with these parameters. Methods In this retrospective cohort study, 3,750 premature infants who underwent ROP screening from 2016 to 2021 were identified from the hospital medical record system. Among 2,130 premature babies screened for firsttrimester aneuploidy, 166 babies whose mothers had single pregnancies were screened by the same method and showed the same clinical course in both eyes were included in the study. The infants were divided into two groups according to the presence of ROP, and those with ROP were further evaluated in two groups according to the presence of proliferation. The groups were compared in terms of the serum values of human chorionic gonadotropin and pregnancy-associated plasma protein A, among aneuploidy screening biomarkers, and NT measurements. Results There was no significant difference in the evaluated serum biomarker values and NT measurements between the ROP and non-ROP groups or between the proliferative ROP, non-proliferative ROP, and non-ROP groups. Conclusion Our results showed that first-trimester aneuploidy screening serum biomarker values and NT measurements were not associated with the development of ROP in premature infants.Öğe The Oxidative and Anti-Angiogenic Effects of Acrylamide in Chorioallantoic Membrane Model(Akdeniz Üniversitesi, 01.05.2023)Monosodium glutamate (MSG) is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG (10-4 M, 10-5 M, and 10-6 M), control, and the bevacizumab (10-6 M) were prepared and placed on the chorioallantoic membrane (CAM) of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group (average score: 0.3). Anti-angiogenic effects were moderate in the 10-5 M MSG group (average score: 0.5) and in the 10-4 M MSG group (average score: 0.7), and strong in the bevacizumab group (average score: 1.1). According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.Öğe Is There a Relationship Between First-Trimester Aneuploidy Screening Serum Biomarker Values and Nuchal Translucency Measurements and the Development of Retinopathy of Prematurity (ROP) in Premature Infants?(Springer, 2023) Küçük, Mehmet Fatih; Savaş Hasan Basri; Süren, Elçin; Erol, Muhammet; Yaprak, Lütfiye; Sabancı, ŞenolPurpose The purpose of this study is the evaluation of serum biomarker and nuchal translucency (NT) values measured during first-trimester aneuploidy screening in terms of the development of retinopathy of prematurity (ROP) in premature infants and investigation of whether the development of ROP is associated with these parameters. Methods In this retrospective cohort study, 3,750 premature infants who underwent ROP screening from 2016 to 2021 were identified from the hospital medical record system. Among 2,130 premature babies screened for first-trimester aneuploidy, 166 babies whose mothers had single pregnancies were screened by the same method and showed the same clinical course in both eyes were included in the study. The infants were divided into two groups according to the presence of ROP, and those with ROP were further evaluated in two groups according to the presence of proliferation. The groups were compared in terms of the serum values of human chorionic gonadotropin and pregnancy-associated plasma protein A, among aneuploidy screening biomarkers, and NT measurements. Results There was no significant difference in the evaluated serum biomarker values and NT measurements between the ROP and non-ROP groups or between the proliferative ROP, non-proliferative ROP, and non-ROP groups. Conclusion Our results showed that first-trimester aneuploidy screening serum biomarker values and NT measurements were not associated with the development of ROP in premature infants.Öğe Thiol–disulfide balance and trace element levels in patients with seasonal allergic rhinitis(African Health Sciences, 2022) Savas, Hasan Basri; Gunizi, HuseyinAbstract Background: The prevalence of allergic diseases is gradually increasing worldwide. The most common such allergic disease is allergic rhinitis (AR). Objective: The present study investigated the possible relationship between seasonal AR and the thiol-disulfide balance and zinc and copper levels in adult individuals. Study Design and Methods: 130 male and female adults were included in the study. The participants’ serum thiol-disulfide balance and zinc and copper levels were measured spectrophotometrically using commercial kits. Statistical significance was accepted as p < 0.05 between the groups. Results: The serum copper (p = 0.001), native thiol (p = 0.006), reduced thiol (p < 0.001), and thiol oxidation reduction ratio (p < 0.001) levels were significantly lower in the seasonal AR group than in the control group. Conclusion: In AR patients, the low level of copper, which is an important trace element, the deterioration of the thiol-disulfide balance, which represents a unique indicator of the oxidant-antioxidant balance, the increased disulfide level caused by oxidative stress, and the decreased native thiol level can all serve as important biochemical markers.
