Effects of tocilizumab and dexamethasone on the downregulation of proinflammatory cytokines and upregulation of antioxidants in the lungs in oleic acid-induced ARDS
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
BMC
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Abstract
Background: Acute respiratory distress syndrome (ARDS) is a life-threatening disease caused by the induction of
infammatory cytokines and chemokines in the lungs. There is a dearth of drug applications that can be used to
prevent cytokine storms in ARDS treatment. This study was designed to investigate the efects of tocilizumab and
dexamethasone on oxidative stress, antioxidant parameters, and cytokine storms in acute lung injury caused by oleic
acid in rats.
Methods: Adult male rats were divided into fve groups: the CN (healthy rats, n=6), OA (oleic acid administration,
n=6), OA+TCZ-2 (oleic acid and tocilizumab at 2 mg/kg, n=6), OA+TCZ-4 (oleic acid and tocilizumab at 4 mg/kg,
n=6), and OA+DEX-10 (oleic acid and dexamethasone at 10 mg/kg, n=6) groups. All animals were euthanized after
treatment for histopathological, immunohistochemical, biochemical, PCR, and SEM analyses.
Results: Expressions of TNF-α, IL-1β, IL-6, and IL-8 cytokines in rats with acute lung injury induced by oleic acid were
downregulated in the TCZ and DEX groups compared to the OA group (P<0.05). The MDA level in lung tissues was
statistically lower in the OA+TCZ-4 group compared to the OA group. It was further determined that SOD, GSH, and
CAT levels were decreased in the OA group and increased in the TCZ and DEX groups (P<0.05). Histopathological
fndings such as thickening of the alveoli, hyperemia, and peribronchial cell infltration were found to be similar when
lung tissues of the TCZ and DEX groups were compared to the control group. With SEM imaging of the lung tissues, it
was found that the alveolar lining layer had become indistinct in the OA, OA+TCZ-2, and OA+TCZ-4 groups.
Conclusions: In this model of acute lung injury caused by oleic acid, tocilizumab and dexamethasone were efective
in preventing cytokine storms by downregulating the expression of proinfammatory cytokines including TNF-α, IL-1β,
IL-6, and IL-8. Against the downregulation of antioxidant parameters such as SOD and GSH in the lung tissues caused
by oleic acid, tocilizumab and dexamethasone upregulated them and showed protective efects against cell damage.
Açıklama
Anahtar Kelimeler
ARDS, Tocilizumab, Dexamethasone, Oleic acid, Rat
Kaynak
Respiratory Research
WoS Q Değeri
Q1
Scopus Q Değeri
N/A
Cilt
23
Sayı
1
Künye
Terzi, F., Demirci, B., Çınar, İ., Alhilal, M., & Erol, H. S. (2022). Effects of tocilizumab and dexamethasone on the downregulation of proinflammatory cytokines and upregulation of antioxidants in the lungs in oleic acid-induced ARDS. Respiratory Research, 23(1), 1-11.
Bağlantı
https://doi.org/10.1186/s12931-022-02172-w
https://pubmed.ncbi.nlm.nih.gov/36115998/
https://www.scopus.com/record/display.uri?eid=2-s2.0-85138206381&origin=resultslist&sort=plf-f&src=s&st1=10.1186%2fs12931-022-02172-w&sid=9397adc674edcd1429d7a3bb6d915b60&sot=b&sdt=b&sl=31&s=DOI%2810.1186%2fs12931-022-02172-w%29&relpos=0&citeCnt=1&searchTerm=
https://hdl.handle.net/20.500.12514/3180
https://www.webofscience.com/wos/woscc/full-record/WOS:000854850600001?AlertId=d383397b-4355-449e-9419-70f9e0e77c15&SID=EUW1ED0E39ELwQcf8Mw3SbSiv2Y5U
https://pubmed.ncbi.nlm.nih.gov/36115998/
https://www.scopus.com/record/display.uri?eid=2-s2.0-85138206381&origin=resultslist&sort=plf-f&src=s&st1=10.1186%2fs12931-022-02172-w&sid=9397adc674edcd1429d7a3bb6d915b60&sot=b&sdt=b&sl=31&s=DOI%2810.1186%2fs12931-022-02172-w%29&relpos=0&citeCnt=1&searchTerm=
https://hdl.handle.net/20.500.12514/3180
https://www.webofscience.com/wos/woscc/full-record/WOS:000854850600001?AlertId=d383397b-4355-449e-9419-70f9e0e77c15&SID=EUW1ED0E39ELwQcf8Mw3SbSiv2Y5U
