Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant
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Tarih
2017
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Erişim Hakkı
Özet
Bu çalışmamızda Acanthus dioscoridis L. var. dioscoridis L.' in toprak üstü kısımlarının hekzan (HEG), etilasetat (EtOAc) ve metanol özütlerinin bazı in vitro biyolojik aktiviteleri detaylı olarak incelenmiştir. Bitki özütlerinin in vitro antioksidan aktiviteleri total fenolik ve flavonoid içerikleri, DPPH serbest radikal söndürücü, metal şelatlama ve indirgeme gücü aktiviteleri bakımından test edildi. MeOH özütünün toplam fenolik madde içeriği 71.18 ?g GAE/mg olarak tespit edilmiştir. En yüksek flavonoid miktarı EtOAc özütünde tespit edilmiştir. En yüksek DPPH radikali söndürme aktivitesi ve indirgeme gücü aktivitesi MeOH özütünde belirlenmiştir. Acanthus dioscoridis L. var. dioscoridis L.' nin HEG özütü diğer özütler ile karşılaştırıldığında açık bir şekilde daha yüksek metal şelatlama aktivitesi göstermiştir.SPUFA yüzdesi anlamlı olarak SMUFA ve SSFA' dan daha yüksekti. Yüksek PUFA içeriği ve güçlü antioksidan aktiviteleri göz önüne alındığında A. dioscoridis' in tıbbi bilimlerde, kozmetik ve gıda endüstrisinde olası kullanımı önerilebilir
In our study, some in vitro biological activities of hexane (HEG), ethylacetate (EtOAc) and methanol (MeOH) extracts of Acanthus dioscoridis L. var. dioscoridis L. aerial parts have been investigated in details. Plant extracts were tested in view of its in vitro antioxidant activities as total phenolic and total flavonoid contents, DPPH free radical-scavenging, metal chelating and reducing power activity. Total phenolic content of MeOH extract has been found as 71.18 ?g GAE/mg. The highest amount of flavonoids has been detected in EtOAc extract. The highest DPPH radical-scavenging activity and reducing power activity has been determined in MeOH extract. HEG extracts of Acanthus dioscoridis has showed clearly higher metal chelating activity when compared to other extracts. SPUFA percentage was meaningfully higher than SMUFA and SSFA. By considering the high PUFA content and strong antioxidant activities it could be concluded that A. dioscoridis could be recommended to possible use in medical science, cosmetic and food industry
In our study, some in vitro biological activities of hexane (HEG), ethylacetate (EtOAc) and methanol (MeOH) extracts of Acanthus dioscoridis L. var. dioscoridis L. aerial parts have been investigated in details. Plant extracts were tested in view of its in vitro antioxidant activities as total phenolic and total flavonoid contents, DPPH free radical-scavenging, metal chelating and reducing power activity. Total phenolic content of MeOH extract has been found as 71.18 ?g GAE/mg. The highest amount of flavonoids has been detected in EtOAc extract. The highest DPPH radical-scavenging activity and reducing power activity has been determined in MeOH extract. HEG extracts of Acanthus dioscoridis has showed clearly higher metal chelating activity when compared to other extracts. SPUFA percentage was meaningfully higher than SMUFA and SSFA. By considering the high PUFA content and strong antioxidant activities it could be concluded that A. dioscoridis could be recommended to possible use in medical science, cosmetic and food industry
Açıklama
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Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi
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Cilt
7
Sayı
2
