Presence of Biofilm and Adhesin Genes in Staphylococcus aureus Strains Taken from Chronic Wound Infections and their Genotypic and Phenotypic Antimicrobial Sensitivity Patterns
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Photodiagnosis and Photodynamic Therapy
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to investigate some biofilm (icaA, icaD and bap) and some adhesion (clfA, fnbA, cna) genes, and also evaluate the phenotypic and genotypic antimicrobial resistance patterns of Staphylococcus aureus strains isolated from wound samples in Mardin, Turkey. A total of 220 wound samples were studied. The biofilm forming ability and resistance pattern for eleven antimicrobial agents were investigated by conventional and multiplex PCR methods. S. aureus was isolated from 112 (50.9%) of 220 wound samples. Moreover, biofilm production was found in 79 (70.5%) of the 112 S. aureus isolates. 97 (86.6%) strains of all isolates were positive for icaA and icaD, and 15 (13.4%) for bap. The adhesin genes, cna, fnbA and clfA were detected in 98 (87.5%), 87 (77.7%), and 75 (66.9%) strains, respectively. The numbers of MSSA and MRSA carrying antimicrobial resistance genes were 19 (16.96%) and 32 (28.57%) for blaZ, 9 (8.04%) and 17 (15.18%) for tetK, 6 (5.36%) and 14 (12.5%) for ermC, 2 (1.79%) and 7 (6.25%) for tetM, 0 (0%) and 5 (4.46%) for mecA, 2 (1.79%) and 4 (3.57%) for ermA, 1 (0.89%) and 2 (1.79 %) for both tetK and tetM, respectively. Our findings indicate that multiplex PCR is a reliable method for identifying biofilm and adhesin producing S. aureus. Our data also provided a nationwide surveillance of the antimicrobial resistance gene profiles of S. aureus for the accurate treatment of patients and to control the dissemination of the resistance genes.
Açıklama
Anahtar Kelimeler
Biofilm gene, adhesin gene, Staphylococcus aureus, Staphylococcus aureus biofilm gene adhesin gene wound infection
Kaynak
Photodiagnosis and Photodynamic Therapy
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
Sayı
Künye
Demir C, Demirci M, Yigin A, Tokman HB, Cetik Yildiz S. Presence of biofilm and adhesin genes in Staphylococcus aureus strains taken from chronic wound infections and their genotypic and phenotypic antimicrobial sensitivity patterns. Photodiagnosis Photodyn Ther. 2020 Mar;29:101584. doi: 10.1016/j.pdpdt.2019.101584. Epub 2019 Nov 2. PMID: 31689511.
