Maltose Functionalized Magnetic Core/Shell Fe3O4@Au Nanoparticles for An Efficient L-Asparaginase Immobilization
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
International Journal of Biological Macromolecules
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, maltose-functionalized magnetic core/shell nanoparticles (Fe3O4@Au NPs) as a
promising carrier matrix for a simple and effective immobilization of L-asparaginase (L
ASNase) were prepared and characterized using imaging techniques including atomic force
microscopy (AFM) and transmission electron microscopy (TEM), and vibrating sample
magnetometry (VSM). The results indicate that the NPs are monodispersed with an average
diameter of 10 nm and magnetization of 9.0 emu g-1. Under the optimal conditions, 77.2 ± 2.3%
of the total L-ASNase was immobilized. It was found that the acid-base tolerance and thermal
stability of immobilized L-ASNase were significantly improved in comparison to the free form
of the enzyme in solution. For instance, while only 10% of the immobilized enzyme was lost
its activity, the free form was lost its activity more than 50% after 3 h incubation at 55 oC. After
13 times recycling, the immobilized L-ASNase retained about 50% of its initial activity.
Moreover, the free and immobilized L-ASNase maintained their initial activities about 25 and
64% after 28 days storage at 25 °C, respectively. Km value of immobilized L-ASNase
decreased to 1.59 from 2.95 mM as an indication of increased enzyme affinity for the substrate.
The results of this study suggest that the maltose-coated magnetic nanoparticles are excellent
nanovehicles to carry enzymes for a range of industrial applications.
Açıklama
Anahtar Kelimeler
Magnetic Core/Shell Nanoparticles, L-Asparaginase, Immobilization, Maltose
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Tarhan T, Ulu A, Sariçam M, Çulha M, Ates B. Maltose functionalized magnetic core/shell Fe3O4@Au nanoparticles for an efficient l-asparaginase immobilization. Int J Biol Macromol. 2020 Jan 1;142:443-451. doi: 10.1016/j.ijbiomac.2019.09.116. Epub 2019 Oct 5. PMID: 31593716.
