Synthesis and characterization of silver nanoparticles decorated polydopamine coated hexagonal boron nitride and its effect on wound healing
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Journal of Trace Elements in Medicine and Biology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: Wound healing is an essential physiological process involving many cell types and their products acting in a marvellous harmony to repair damaged tissues. During the healing process, cellular proliferation and extracellular matrix remodelling stages could be interrupted by undesired factors including microorganisms and altered metabolic activities. In such a case, the process requires some external stimulants to accelerate or remediate the healing stages.
Methods: In this study, we report a multifunctional wound healing stimulating agent. In this context, hexagonal boron nitride (hBN) nanoparticles, silver nanoparticles (AgNPs) and polydopamine(pdopa) were used through mussel-inspired chemistry of dopamine to obtain pdopa coated hBN (hBN@pdopa) and AgNPs decorated hBN@pdopa (hBN@pdopa-AgNPs). These two nanostructures were investigated to observe stages of healing.
Results: AgNPs were chosen for inflammation reduction and hBN for induced cell proliferation and migration. In in vitro experiments, firstly, high cellular uptake capacity and biocompatibility of hBN@pdopa and hBN@pdopa-AgNPs were evaluated. They were also tested for their reaction against increased concentration of reactive oxygen species (ROS) in injured cells. Finally, their effect on cellular migration, intracellular tube formation and F-actin organization were monitored by light and confocal microscopy, respectively.
Conclusion: The results clearly indicate that the hBN@pdopa-AgNPs significantly decrease ROS production, promote wound closure, and reorganize tube formation in cells.
Açıklama
Anahtar Kelimeler
Angiogenesis; Biocompatibility; Hexagonal boron nitrides; Polydopamine; Silver nanoparticles; Wound healing.
Kaynak
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
67
Sayı
Künye
Tarhan, T., Şen, Ö., Ciofani, M. E., Yılmaz, D., & Çulha, M. (2021). Synthesis and characterization of silver nanoparticles decorated polydopamine coated hexagonal boron nitride and its effect on wound healing. In Journal of Trace Elements in Medicine and Biology (Vol. 67, p. 126774). Elsevier BV. https://doi.org/10.1016/j.jtemb.2021.126774
