Investigation of the antibiotic resistance and biofilm-forming ability of Staphylococcus aureus from subclinical bovine mastitis cases

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dc.contributor.authorAslantas, Ozkan
dc.contributor.authorDemir, Cemil
dc.date.accessioned14.07.201910:50:10
dc.date.accessioned2019-07-16T20:43:59Z
dc.date.available14.07.201910:50:10
dc.date.available2019-07-16T20:43:59Z
dc.date.issued2016
dc.departmentMAÜ, Meslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.description.abstractA total of 112 Staphylococcus aureus isolates obtained from subclinical bovine mastitis cases were examined for antibiotic susceptibility and biofilm-forming ability as well as genes responsible for antibiotic resistance, biofilm-forming ability, and adhesin. Antimicrobial susceptibility of the isolates were determined by disk diffusion method. Biofilm forming ability of the isolates were investigated by Congo red agar method, standard tube method, and microplate method. The genes responsible for antibiotic resistance, biofilm-forming ability, and adhesion were examined by PCR. Five isolates (4.5%) were identified as methicillin-resistant Staph. aureus by antibiotic susceptibility testing and confirmed by mecA detection. The resistance rates to penicillin, ampicillin, tetracycline, erythromycin, trimethoprim-sulfamethoxazole, enrofloxacin, and amoxicillin-clavulanic acid were 45.5, 39.3, 33, 26.8, 5.4, 0.9, and 0.9%, respectively. All isolates were susceptible against vancomycin and gentamicin. The blaZ (100%), tetK (67.6%), and ermA (70%) genes were the most common antibiotic-resistance genes. Using Congo red agar, microplate, and standard tube methods, 70.5, 67, and 62.5% of the isolates were found to be biofilm producers, respectively. The percentage rate of icaA, icaD, and bap genes in Staph. aureus isolates were 86.6, 86.6, and 13.4%, respectively. The adhesion molecules fnbA, can, and clfA were detected in 87 (77.7%), 98 (87.5%), and 75 (70%) isolates, respectively. The results indicated that Staph. aureus from sublinical bovine mastitis cases were mainly resistant to beta-lactams and, to a lesser extent, to tetracycline and erythromycin. Also, biofilm- and adhesion-related genes, which are increasingly accepted as an important virulence factor in the pathogenesis of Staph. aureus infections, were detected at a high rate.en_US
dc.description.sponsorshipMustafa Kemal University Research Fund [08 L 0602]en_US
dc.description.sponsorshipThe authors thank Mustafa Kemal University Research Fund (Project Number: 08 L 0602) for financially supporting this study.en_US
dc.identifier.citationAslantaş Ö, Demir C. Investigation of the antibiotic resistance and biofilm-forming ability of Staphylococcus aureus from subclinical bovine mastitis cases. J Dairy Sci. 2016 Nov;99(11):8607-8613. doi: 10.3168/jds.2016-11310. Epub 2016 Aug 31. PMID: 27592437.
dc.identifier.doi10.3168/jds.2016-11310en_US
dc.identifier.endpage8613en_US
dc.identifier.issn0022-0302
dc.identifier.issn1525-3198
dc.identifier.issue11en_US
dc.identifier.pmid27592437en_US
dc.identifier.scopus2-s2.0-84994187821en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage8607en_US
dc.identifier.urihttps://dx.doi.org/10.3168/jds.2016-11310
dc.identifier.urihttps://hdl.handle.net/20.500.12514/1329
dc.identifier.volume99en_US
dc.identifier.wosWOS:000385610700011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.ispartofJOURNAL OF DAIRY SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectantibiotic resistanceen_US
dc.subjectbiofilm productionen_US
dc.subjectmastitisen_US
dc.subjectStaphylococcus aureusen_US
dc.titleInvestigation of the antibiotic resistance and biofilm-forming ability of Staphylococcus aureus from subclinical bovine mastitis casesen_US
dc.typeArticleen_US

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