The interaction methylene blue and glutathione-S-transferase purified from human erythrocytes

Yükleniyor...
Küçük Resim

Tarih

2020

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Journal of Planar Chromatography - Modern TLC

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

It is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification and 30% efficiency by glutathione agarose affinity chromatography. The results showed that the enzyme was inhibited by methylene blue with an IC50 value of 1.40 mmol/L. The Ki constant of methylene blue was 1.17 mmol/L. The Lineweaver–Burk graph of the methylene blue showed that the type of inhibition was compatible with mixed type inhibition. A new third spot was also detected by thin-layer chromatography. Furthermore, the cytotoxicity of methylene blue on human erythrocytes was evaluated and it was found that the haemolysis per cent of methylene blue on erythrocytes was approximately 14%.

Açıklama

Anahtar Kelimeler

Glutathione-S-transferase . Methylene blue . Thin-layer chromatography . Affinity chromatography

Kaynak

WoS Q Değeri

Q4

Scopus Q Değeri

N/A

Cilt

33

Sayı

3

Künye

Aygun, H.; Department of Biology, Faculty of Science, Dicle University, Diyarbakir, Turkey.