The interaction methylene blue and glutathione-S-transferase purified from human erythrocytes

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dc.contributor.authorAcay, Hilal
dc.contributor.authorUzan, Serhat
dc.contributor.authorBaran, Mehmet Fırat
dc.contributor.authorBilden, Alican
dc.contributor.authorAygün, Hüsamettin
dc.date.accessioned2021-10-30T14:43:35Z
dc.date.available2021-10-30T14:43:35Z
dc.date.issued2020en_US
dc.departmentMAÜ, Fakülteler, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümüen_US
dc.description.abstractIt is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification and 30% efficiency by glutathione agarose affinity chromatography. The results showed that the enzyme was inhibited by methylene blue with an IC50 value of 1.40 mmol/L. The Ki constant of methylene blue was 1.17 mmol/L. The Lineweaver–Burk graph of the methylene blue showed that the type of inhibition was compatible with mixed type inhibition. A new third spot was also detected by thin-layer chromatography. Furthermore, the cytotoxicity of methylene blue on human erythrocytes was evaluated and it was found that the haemolysis per cent of methylene blue on erythrocytes was approximately 14%.en_US
dc.identifier.citationAygun, H.; Department of Biology, Faculty of Science, Dicle University, Diyarbakir, Turkey.en_US
dc.identifier.doi10.1007/s00764-020-00030-3en_US
dc.identifier.endpage269en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85086788493en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage263en_US
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85086788493&doi=10.1007%2fs00764-020-00030-3&origin=inward&txGid=0832669ef3d3615f6e45fcd42d07387b&featureToggles=FEATURE_VIEW_PDF:1
dc.identifier.urihttps://hdl.handle.net/20.500.12514/2917
dc.identifier.volume33en_US
dc.identifier.wosWOS:000542927000001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherJournal of Planar Chromatography - Modern TLCen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGlutathione-S-transferase . Methylene blue . Thin-layer chromatography . Affinity chromatographyen_US
dc.titleThe interaction methylene blue and glutathione-S-transferase purified from human erythrocytesen_US
dc.typeArticleen_US

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