Investigate Nasal Colonize Staphylococcus Species Biofilm Produced
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Tarih
2015
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Özet
Amaç: Hastaların, burunlarından izole edilen, 127 S. aureus ve 65 KNS (koagulaz negatif stafilokok) suşunun, biyofilm oluşturma yeteneği üç farklı yöntem kullanılarak araştırıldı. Biyofilm pozitif ve negatif stafilokokların, farklı antibiyotiklere karşı olan dirençlilikleri değerlendirildi. Gereç ve Yöntem: Alı- nan eküvyon örnekleri, % 7’lik kanlı agara ekildi. Staphylococcus aureus ve KNS izolatları, biyofilm üretme yetenekleri, Congo Red Agar (CRA), Mikroplak (MP) ve Standart Tüp (ST) yöntemleri kullanılarak araştırıldı. Ayrıca, stafilokok suşlarının, çeşitli antibiyotiklere karşı olan dirençlilikleri, agar disk difüzyon yöntemi ile araştırıldı. Bulgular: İncelenen stafilokok suşlarının, CRA, MP ve ST testleri ile biyofilm üretimi sırasıyla, %72,4, %67,7 ve %62,9 oranında pozitif bulundu. CRA, MP ve ST testleri arasında, istatistiksel olarak, anlamlı farklılık saptanmadı (p>0,05). Biyofilm pozitif (BP) stafilokok suşlarında, penisilin G, ampisilin, amoksisilin/klavulanik asit, tetrasiklin, eritromisin, gentamisin, enrofloksasine olan direnç oranları sırasıyla %71,7, %69,7, %6,2, %20 ,7, %21,4, %1,4, %0,7 , Biyofilm negatif (BN) stafilokok suşlarında ise, %42,6, %23,4, %4,3, % 14,9, %19,1, %0,0, 0,0% olarak tespit edildi. İncelenen tüm stafilokoklar ise, vankomisin ve teikoplanine duyarlı bulundu. Antibiyotiklere dirençlilik oranları, BP suşlarda BN suşlara göre daha yüksek bulunmakla birlikte, dirençlilik oranları istatistiksel olarak anlamlı bulunamadı (p>0,05). Tartışma: Stafilokok suşlarında, biyofilm üretiminin belirlenmesinde CRA testinin daha pratik ve spesifik olduğu, antibiyotiklere direnç gelişiminde de, biyofilm üretiminin önemli rol oynadığı sonucuna varıldı.
Aim: 127 S.aureus and 65 CoNS strains were isolated from patients noses’. To produce a biofilm ability was investigated using three different methods. Slime-positive and negative staphylococcies’ resistance were evaluated against different antibiotics. Material and Method: Swap samples puted 7% blood agar. Staphylococcus aureus and coagulase-negative staphylococci (CoNS) isolates biofilm produced ability were investigated using Congo Red Agar (CRA), microplates (MP) and Standard Tube (ST) methods. In addition to that, presence of antibiotic resistance of the staphylococcal isolates are determined agar disc diffusion method. Results: The rate of biofilm producing Staphylococcus spp strains was found to be 72.4%, 67.7%, and 62.9%, respectively with CRA, MP, and ST tests. There was no significant relationship among the tests (p>0.05). In addition, antibiotic resistance of Staphylococcus spp. against various antibiotics was also determined by the agar disk diffusion method. Resistance rates of biofilm positive (BP) Staphylococcus spp for penicilin G, ampicilin, amocycilin/clavulanic acid, tetracyclin, eritromycin, gentamycin, and enrofloxacin 71.7%, 69.7%, 6.2%, 20.7%, 21.4%, 1.4%, and 0.7%, respectively. Resistance rates of biofilm negative (BN) spp for 42.6%, 23.4%, 4.3%, 14.9%, 19.1%, 0.0%, 0.0% respectively. All Staphylococcus isolates were found to be susceptible to vancomycin and teicaplonin. Although BP strains antibiotic resistance rates were observed higher than BN strains. But resistance rates were not found statistically significant (p>0.05). Discussion: CRA is the reliablity and specifity method to determine Staphylococcus spp. biofilm produce ability.
Aim: 127 S.aureus and 65 CoNS strains were isolated from patients noses’. To produce a biofilm ability was investigated using three different methods. Slime-positive and negative staphylococcies’ resistance were evaluated against different antibiotics. Material and Method: Swap samples puted 7% blood agar. Staphylococcus aureus and coagulase-negative staphylococci (CoNS) isolates biofilm produced ability were investigated using Congo Red Agar (CRA), microplates (MP) and Standard Tube (ST) methods. In addition to that, presence of antibiotic resistance of the staphylococcal isolates are determined agar disc diffusion method. Results: The rate of biofilm producing Staphylococcus spp strains was found to be 72.4%, 67.7%, and 62.9%, respectively with CRA, MP, and ST tests. There was no significant relationship among the tests (p>0.05). In addition, antibiotic resistance of Staphylococcus spp. against various antibiotics was also determined by the agar disk diffusion method. Resistance rates of biofilm positive (BP) Staphylococcus spp for penicilin G, ampicilin, amocycilin/clavulanic acid, tetracyclin, eritromycin, gentamycin, and enrofloxacin 71.7%, 69.7%, 6.2%, 20.7%, 21.4%, 1.4%, and 0.7%, respectively. Resistance rates of biofilm negative (BN) spp for 42.6%, 23.4%, 4.3%, 14.9%, 19.1%, 0.0%, 0.0% respectively. All Staphylococcus isolates were found to be susceptible to vancomycin and teicaplonin. Although BP strains antibiotic resistance rates were observed higher than BN strains. But resistance rates were not found statistically significant (p>0.05). Discussion: CRA is the reliablity and specifity method to determine Staphylococcus spp. biofilm produce ability.
Açıklama
Anahtar Kelimeler
Biofilm
Kaynak
Journal of Clinical and Analytical Medicine
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
6
Sayı
4
