Synthesis and characterization of magnetic nanocomposite for in vitro evaluation of irinotecan using human cell lines
Yükleniyor...
Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Journal of Chemistry
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, magnetic O-carboxymethyl chitosan (MOCC) nanocomposite was synthesized and characterized as a drug
delivery system for loading the anticancer drug irinotecan (CPT-11). To increase the drug loading capacity, MOCC was synthesized by
linking the carboxyl group functionally to chitosan. Also, several critical factors such as concentration, the dose of MOCC, and contact
time for optimum drug loading condition were investigated. The loading capacity of CPT-11 onto MOCC was calculated as 5.6 mg/g,
and the loaded drug concentration was calculated as 0.04787 mM at pH value of 5. Besides, the cytotoxic properties of MOCC, CPT11 loaded MOCC (MOCC-CPT-11), and free CPT-11 were studied on glioblastoma multiforme cell lines, including U87 and U373.
According to the results, the MOCC-CPT-11 showed at least as toxic effect as free CPT-11 even at very low concentrations, while the
MOCC showed slight toxicity (cell viability of 96% to 78%) on U373 cell lines at all concentrations and for 24 h and 48 h incubation
times. Moreover, the results showed that the MOCC indicated significant toxicity in increasing concentrations and incubation times,
and the MOCC-CPT-11 is as toxic as free CPT-11 on U87 cells at all concentrations and incubation times.
Açıklama
Anahtar Kelimeler
Drug nanocarrier, magnetic nanocomposite, cytotoxicity, glioblastoma multiforme, irinotecan
Kaynak
Turkish Journal of Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
45
Sayı
3
Künye
Tarhan, T. (2021). Synthesis and characterization of magnetic nanocomposite for in vitro evaluation of irinotecan using human cell lines. TURKISH JOURNAL OF CHEMISTRY, 45(3) p. 540-550, DOI10.3906/kim-2010-53
Bağlantı
https://www.webofscience.com/wos/woscc/full-record/WOS:000668365800004?AlertId=d383397b-4355-449e-9419-70f9e0e77c15&SID=E5vn6BrBu1Ue95ENxH3
https://journals.tubitak.gov.tr/chem/abstract.htm?id=29428
https://hdl.handle.net/20.500.12514/2713
https://pubmed.ncbi.nlm.nih.gov/34385850/
https://www.scopus.com/record/display.uri?eid=2-s2.0-85110979679&origin=resultslist&sort=plf-f&src=s&sid=ccfcdeb1af3b0eb9510cd96823d5e071&sot=b&sdt=b&sl=24&s=DOI%2810.3906%2fkim-2010-53%29&relpos=0&citeCnt=0&searchTerm=
https://journals.tubitak.gov.tr/chem/abstract.htm?id=29428
https://hdl.handle.net/20.500.12514/2713
https://pubmed.ncbi.nlm.nih.gov/34385850/
https://www.scopus.com/record/display.uri?eid=2-s2.0-85110979679&origin=resultslist&sort=plf-f&src=s&sid=ccfcdeb1af3b0eb9510cd96823d5e071&sot=b&sdt=b&sl=24&s=DOI%2810.3906%2fkim-2010-53%29&relpos=0&citeCnt=0&searchTerm=